anti albumin sheep polyclonal antibody  (Bio-Rad)

Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Detection of a large antigen through the masking and exposure of a fragment of split luciferase.

doi: 10.1007/s44211-025-00754-4

Figure Lengend Snippet: Fig. 2 Design, preparation, and characterization of the PMBiT probe. a Design and the preparation of PMBiT. TrxA: thioredoxin, S: twin-strep-tag, C: cys-tag, L: (G3S)2 linker, H: His-tag. b Binding activity of PM, PM (319 Azide), PMBiT against human polyclonal IgGs by ELISA. c Reconstitution of the luciferase confirmed by bioluminescence. All data are shown as means ± standard deviation (n = 3)

Article Snippet: Goat anti-bovine lactoferrin (A10-126A) and sheep anti-bovine albumin (A10-113A) polyclonal antibodies were purchased from Bethyl Laboratories (TX, USA).

Techniques: Strep-tag, Binding Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Luciferase, Standard Deviation

Journal: BMC Biotechnology

Article Title: Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

doi: 10.1186/1472-6750-9-42

Figure Lengend Snippet: FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from CHO-tPA transfectant pool. B : detection of secreted tPA using an anti-tPA antibody conjugated to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).

Article Snippet: IGF-E5 blots were treated with an anti-His Tag antibody (Serotec) followed by an anti-mouse conjugated to Alexa488 (Invitrogen). tPA blots were treated with a sheep anti-tPA primary antibody (Cedarlane) followed by a rabbit anti-sheep antibody conjugated to Cy3 (Jackson, Immunoresearch Labs, West Grove, PA).

Techniques: Transfection, Light Microscopy

Journal: Molecular immunology

Article Title: The Human Complement Receptor Type 2 (CR2)/CR1 Fusion Protein TT32, a Novel Targeted Inhibitor of the Classical and Alternative Pathway C3 Convertases, Prevents Arthritis in Active Immunization and Passive Transfer Mouse Models

doi: 10.1016/j.molimm.2018.09.013

Figure Lengend Snippet: Effect of TT32 on AP50 and CP50 activities. The AP50 and CP50 haemolytic complement activities were measured by using Rabbit blood cells (RBCs) and Sheep red blood cells lytic assays, respectively. The AH50 and CH50 were determined by analyzing the capacity of human and mouse serum incubated with various doses of TT32 or TT30 or SCR1–10 to lyse antibody coated RBCs or SBCs. The calculated percentage (%) of lysis of the control and serum samples from human and mouse were plotted against dilution factor. TT32 inhibits both human (A, B) and mouse (A) complement activation more efficiently than TT30 or CR1 1–10 using rabbit RBC alternative pathway (A, B) or sheep RBC classical pathway (C) lysis assay. HS, human serum; MS, mouse serum.

Article Snippet: Sensitization of sheep RBC (Bioreclamation, Westbury, NY) was achieved by incubation with rabbit anti-sheep hemolysin (Cedarlane Labs) in the presence of gelatin-veronal buffer (Boston Bioproducts) for 30 min at 37 ° C. Next, the optimal serum concentration to lyse erythrocytes via CP activation was determined by incubating sensitized SRBCs with serial dilutions of complement-preserved human serum and measuring hemoglobin release at 541 nm.

Techniques: Incubation, Lysis, Activation Assay